Microbial and operational response of an anaerobic fixed bed digester to oleic acid overloads

Phylogenetic description of immobilized methanogenic community using real-time PCR in a fixed-bed anaerobic digester.

After immobilization of anaerobes on polyurethane foam in a thermophilic, fixed-bed, anaerobic digester supplied with acetate, the results of real-time PCR analysis indicated that the major immobilized methanogenic archaea were Methanosarcina spp., and that the major free-living methanogenic archaea were Methanosarcina and Methanobacterium spp. 16S rRNA gene densities of Methanosarcina spp. and...

Microbial populations associated with fixed- and floating-bed reactors during a two-stage anaerobic process.

Microbial populations associated with methanogenic fixed- or floating-bed bioreactors used for anaerobic digestion of lignocellulosic waste were investigated. Fluorescent in situ hybridization (FISH) was used to characterize microorganisms in samples obtained from different heights in the reactors, which were operated in a semi-continuous manner (feeding and mixing once every 2 days). The FISH ...

Microbial Community Analysis Using MiSeq Sequencing and Pathway of Methane Production in Tehran WWTP: A Full-Scale Anaerobic Digester

Introduction: One of biological wastewater treatment methods that utilizes to both digesting waste activated sludge and methane production is anaerobic digestion (AD). It is believed to be most effective solution in terms of energy crisis and environmental pollution issues. Materials and Methods: In this study the sludge was digested anaerobically sampled from a full-scale WWTP, located at sou...

Molecular monitoring of microbial diversity in expanded granular sludge bed (EGSB) reactors treating oleic acid.

Abstract A molecular approach was used to evaluate the microbial diversity of bacteria and archaea in two expanded granular sludge bed (EGSB) reactors fed with increasing oleic acid loading rates up to 8 kg of chemical oxygen demand (COD) m(-3) day(-1) as the sole carbon source. One of the reactors was inoculated with granular sludge (RI) and the other with suspended sludge (RII). During operat...

Anaerobic Digester Feasibility Study